Vanderbilt University · PhysiologyStein Insulin Lab
Roland W Stein · Nashville, Tennessee
Research Focus
Dr. Roland W Stein's lab studies the differences between human and rodent islet beta cells to understand how they manage insulin secretion, particularly focusing on a key protein called MAFA. They explore how variations in gene expression and postnatal development affect insulin function and diabetes risk. By using human stem cell-derived models, the lab aims to uncover mechanisms that could lead to better treatments for diabetes and other related conditions.
beta cellshuman insulin secretionMAFAdiabetesislet physiologygene expressionhuman pluripotent stem cellspostnatal development
Research Aims
5AIM 01Investigate the role of MAFA in human islet cell function and insulin regulation.
AIM 02Examine differences in MAFA expression between juvenile and adult human beta cells.
AIM 03Analyze the effects of the pathogenic MAFA variant on human cell activity and glucose metabolism.
AIM 04Explore molecular pathways associated with aging and senescence in beta cells.
AIM 05Utilize human stem cell-derived beta-like cells to model human islet physiology.
Skills the Lab Values
cell culturegene knockout techniquesglucose tolerance testingmolecular cloningstem cell differentiationimmunohistochemistryin vitro insulin secretion assaysanimal model handlingexperimental designdata analysis
Output against the department
Publications157top 47% of dept.134th of 286
this lab
most publishedleast published
Citations12ktop 25% of dept.72nd of 286
this lab
most citedleast cited
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