Stanford University · GeneticsLab for Single-Cell Gene Dynamics
William James Greenleaf · Stanford, California
Research Focus
Dr. William James Greenleaf's lab at Stanford University focuses on understanding complex biological systems using cutting-edge single-cell technologies. The research aims to unravel gene regulatory dynamics, particularly in the developing human heart, which has significant implications for understanding congenital heart defects. By leveraging advanced computational tools and multi-omic data analysis, the team is developing models that predict how genetic variations impact gene expression and cellular behavior during development.
single-cell analysisgene regulationcongenital heart defectsCRISPRmulti-omic datamachine learning3D organoidsRNA-seqATAC-seq
Research Aims
5AIM 01Develop scalable methods for analyzing single-cell multi-omic datasets.
AIM 02Map and model the gene regulatory landscape during heart development.
AIM 03Use CRISPR to perturb transcription factors and study their effects at a single-cell level.
AIM 04Create predictive models of gene regulatory networks based on dynamic data from heart organoids.
AIM 05Identify genetic determinants of congenital heart defects through experimental validation.
Skills the Lab Values
single-cell RNA sequencingCRISPR gene editingATAC-seqdata visualizationcomputational modelingdeep learningcell culturebioinformaticsgenome editing
Output against the department
Publications508top 14% of dept.35th of 259
this lab
most publishedleast published
Citations49ktop 13% of dept.33rd of 259
this lab
most citedleast cited
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