Harvard University · BiologyThe Ubiquitin Dynamics Lab
Jeffrey W Harper · Cambridge, Massachusetts
Research Focus
The lab of Dr. Jeffrey W. Harper at Harvard Medical School focuses on understanding how a class of proteins called Cullin-Ring E3 ubiquitin ligases (CRLs) control various biological processes, including the cell cycle and stem cell differentiation. By using genetic and biochemical methods, the lab aims to uncover how these ligases target specific proteins for degradation, a crucial process for maintaining cellular function and integrity. Their research may reveal important insights into development, aging, and cancer biology, potentially leading to new therapeutic strategies.
ubiquitin ligasesstem cellscell cycleprotein degradationproteomicsgene regulationdifferentiationCRLsbiochemical methods
Research Aims
3AIM 01Identify targets of Cullin-Ring E3 ubiquitin ligases in human stem cells.
AIM 02Investigate the role of N/C-End E3 ligases in proteome remodeling during differentiation.
AIM 03Develop methods for uncovering substrate-degron relationships for CRL adaptors.
Skills the Lab Values
genetic screeningproteomic analysiscell cultureCRISPR techniquesbiochemical assaysWestern blottingmicroscopydata analysis using Pythoncell differentiation techniques