UWMUniversity of Wisconsin-Madison · Engineering
Cardiomyocyte Development and Therapy Lab
Sean P Palecek · Madison, Wisconsin
Research Focus
Dr. Sean P Palecek's research lab focuses on improving the manufacturing processes for human pluripotent stem cell-derived cardiomyocytes, which are essential for developing effective cell therapies for heart disease and other conditions. By using advanced techniques like single-cell profiling, they aim to identify and correct failures in the differentiation process, making it more reliable and cost-effective. The lab works with both 2D and 3D bioreactor systems to enhance the scalability and reproducibility of these important cells.
stem cellscardiomyocytescell therapybioreactorssingle-cell transcriptomicsdrug developmentfeedback-controlled processeshuman pluripotent stem cellsdifferentiation
Research Aims
3AIM 01Identify failure modes in 2D cardiomyocyte differentiation through molecular profiling.
AIM 02Develop and validate closed-loop processes for 2D cardiomyocyte differentiation.
AIM 03Scale up the cardiomyocyte differentiation process to 3D bioreactors and assess variability.
Skills the Lab Values
single-cell RNA sequencing (scRNAseq)transposase-accessible chromatin sequencing (ATAC-seq)process optimizationbioreactor operationgenetic engineeringepigenetic analysisbioinformaticscell culturedata analysis
Output against the department
Publications283top 31% of dept.86th of 278
this lab
most publishedleast published
Citations20ktop 18% of dept.51st of 278
this lab
most citedleast cited
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